recombinant activin a Search Results


human recombinant activin a  (Ajinomoto Althea)
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Ajinomoto Althea human recombinant activin a
Human Recombinant Activin A, supplied by Ajinomoto Althea, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human mouse rat activin  (R&D Systems)
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R&D Systems recombinant human mouse rat activin
Recombinant Human Mouse Rat Activin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ac recombinant human bmp4 r d systems  (R&D Systems)
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activin a  (R&D Systems)
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R&D Systems activin a
Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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activin a  (Proteintech)
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Proteintech activin a
Activin A, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant human activin a  (R&D Systems)
93
R&D Systems recombinant human activin a
Recombinant Human Activin A, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β cell differentiation  (Proteintech)
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Proteintech β cell differentiation
β Cell Differentiation, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant activina  (R&D Systems)
93
R&D Systems human recombinant activina
a , Schematic of Eomes Gfp/Δ (EoKO) and Bra Tom/Δ (BraKO) loss-of-function alleles in mouse embryonic stem cells (ESCs). One allele contains a fluorescent reporter ( Gfp or Tomato , Tom ) knock-in, the second allele contains frame-shift deletions in exon 1 of each gene. b , Differentiation protocol to mesoderm and definitive endoderm (ME) cell types: mouse ESCs are maintained in LIF+2i medium, followed by aggregation to embryoid bodies (EBs) in serum-free (SF-basal) medium for 2 days, and treatment with <t>ActivinA</t> (ActA) from day 2 to day 5. Arrows indicate time points of analysis. c , Relative mRNA expression of ME markers during differentiation of WT, BraKO, EoKO, and double knock-out (dKO) cells measured by qRT-PCR in a 5-day time-course showing the complete absence of ME differentiation in dKO cells. Error bars indicate SEM. p-values for differences of mean expression between WT and dKO samples were calculated by Student’s t-test. *:p≤0.05; **:p≤0.01; ***: p≤0.001; ****: p≤0.0001. d , Immunofluorescence (IF) staining of definitive endoderm (SOX17 and FOXA2) and mesoderm (FOXC2) markers at day 4 of differentiation. Scale bars 100 μm. e , Heatmap showing clustered groups (I to V) of downregulated genes (adjusted p-value≤0.05, log 2 (FC)≤-2.5) in dKO compared to WT cells at day 5 of differentiation analyzed by RNA-seq. Scale indicates centered scaled counts normalized by library size and gene-wise dispersion. Important genes in each group are indicated to the right. f , Gene ontology (GO) enrichment analysis of downregulated genes in dKO compared to WT cells indicating the significantly reduced signatures of ME developmental programs in dKO cells. p-values for each term are indicated.
Human Recombinant Activina, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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afl 338  (R&D Systems)
93
R&D Systems afl 338
a , Schematic of Eomes Gfp/Δ (EoKO) and Bra Tom/Δ (BraKO) loss-of-function alleles in mouse embryonic stem cells (ESCs). One allele contains a fluorescent reporter ( Gfp or Tomato , Tom ) knock-in, the second allele contains frame-shift deletions in exon 1 of each gene. b , Differentiation protocol to mesoderm and definitive endoderm (ME) cell types: mouse ESCs are maintained in LIF+2i medium, followed by aggregation to embryoid bodies (EBs) in serum-free (SF-basal) medium for 2 days, and treatment with <t>ActivinA</t> (ActA) from day 2 to day 5. Arrows indicate time points of analysis. c , Relative mRNA expression of ME markers during differentiation of WT, BraKO, EoKO, and double knock-out (dKO) cells measured by qRT-PCR in a 5-day time-course showing the complete absence of ME differentiation in dKO cells. Error bars indicate SEM. p-values for differences of mean expression between WT and dKO samples were calculated by Student’s t-test. *:p≤0.05; **:p≤0.01; ***: p≤0.001; ****: p≤0.0001. d , Immunofluorescence (IF) staining of definitive endoderm (SOX17 and FOXA2) and mesoderm (FOXC2) markers at day 4 of differentiation. Scale bars 100 μm. e , Heatmap showing clustered groups (I to V) of downregulated genes (adjusted p-value≤0.05, log 2 (FC)≤-2.5) in dKO compared to WT cells at day 5 of differentiation analyzed by RNA-seq. Scale indicates centered scaled counts normalized by library size and gene-wise dispersion. Important genes in each group are indicated to the right. f , Gene ontology (GO) enrichment analysis of downregulated genes in dKO compared to WT cells indicating the significantly reduced signatures of ME developmental programs in dKO cells. p-values for each term are indicated.
Afl 338, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stemfit basic 04 complete type medium  (Ajinomoto Althea)
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Ajinomoto Althea stemfit basic 04 complete type medium
a , Schematic of Eomes Gfp/Δ (EoKO) and Bra Tom/Δ (BraKO) loss-of-function alleles in mouse embryonic stem cells (ESCs). One allele contains a fluorescent reporter ( Gfp or Tomato , Tom ) knock-in, the second allele contains frame-shift deletions in exon 1 of each gene. b , Differentiation protocol to mesoderm and definitive endoderm (ME) cell types: mouse ESCs are maintained in LIF+2i medium, followed by aggregation to embryoid bodies (EBs) in serum-free (SF-basal) medium for 2 days, and treatment with <t>ActivinA</t> (ActA) from day 2 to day 5. Arrows indicate time points of analysis. c , Relative mRNA expression of ME markers during differentiation of WT, BraKO, EoKO, and double knock-out (dKO) cells measured by qRT-PCR in a 5-day time-course showing the complete absence of ME differentiation in dKO cells. Error bars indicate SEM. p-values for differences of mean expression between WT and dKO samples were calculated by Student’s t-test. *:p≤0.05; **:p≤0.01; ***: p≤0.001; ****: p≤0.0001. d , Immunofluorescence (IF) staining of definitive endoderm (SOX17 and FOXA2) and mesoderm (FOXC2) markers at day 4 of differentiation. Scale bars 100 μm. e , Heatmap showing clustered groups (I to V) of downregulated genes (adjusted p-value≤0.05, log 2 (FC)≤-2.5) in dKO compared to WT cells at day 5 of differentiation analyzed by RNA-seq. Scale indicates centered scaled counts normalized by library size and gene-wise dispersion. Important genes in each group are indicated to the right. f , Gene ontology (GO) enrichment analysis of downregulated genes in dKO compared to WT cells indicating the significantly reduced signatures of ME developmental programs in dKO cells. p-values for each term are indicated.
Stemfit Basic 04 Complete Type Medium, supplied by Ajinomoto Althea, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Schematic of Eomes Gfp/Δ (EoKO) and Bra Tom/Δ (BraKO) loss-of-function alleles in mouse embryonic stem cells (ESCs). One allele contains a fluorescent reporter ( Gfp or Tomato , Tom ) knock-in, the second allele contains frame-shift deletions in exon 1 of each gene. b , Differentiation protocol to mesoderm and definitive endoderm (ME) cell types: mouse ESCs are maintained in LIF+2i medium, followed by aggregation to embryoid bodies (EBs) in serum-free (SF-basal) medium for 2 days, and treatment with ActivinA (ActA) from day 2 to day 5. Arrows indicate time points of analysis. c , Relative mRNA expression of ME markers during differentiation of WT, BraKO, EoKO, and double knock-out (dKO) cells measured by qRT-PCR in a 5-day time-course showing the complete absence of ME differentiation in dKO cells. Error bars indicate SEM. p-values for differences of mean expression between WT and dKO samples were calculated by Student’s t-test. *:p≤0.05; **:p≤0.01; ***: p≤0.001; ****: p≤0.0001. d , Immunofluorescence (IF) staining of definitive endoderm (SOX17 and FOXA2) and mesoderm (FOXC2) markers at day 4 of differentiation. Scale bars 100 μm. e , Heatmap showing clustered groups (I to V) of downregulated genes (adjusted p-value≤0.05, log 2 (FC)≤-2.5) in dKO compared to WT cells at day 5 of differentiation analyzed by RNA-seq. Scale indicates centered scaled counts normalized by library size and gene-wise dispersion. Important genes in each group are indicated to the right. f , Gene ontology (GO) enrichment analysis of downregulated genes in dKO compared to WT cells indicating the significantly reduced signatures of ME developmental programs in dKO cells. p-values for each term are indicated.

Journal: bioRxiv

Article Title: Eomes and Brachyury control pluripotency exit and germ layer segregation by changes of chromatin state

doi: 10.1101/774232

Figure Lengend Snippet: a , Schematic of Eomes Gfp/Δ (EoKO) and Bra Tom/Δ (BraKO) loss-of-function alleles in mouse embryonic stem cells (ESCs). One allele contains a fluorescent reporter ( Gfp or Tomato , Tom ) knock-in, the second allele contains frame-shift deletions in exon 1 of each gene. b , Differentiation protocol to mesoderm and definitive endoderm (ME) cell types: mouse ESCs are maintained in LIF+2i medium, followed by aggregation to embryoid bodies (EBs) in serum-free (SF-basal) medium for 2 days, and treatment with ActivinA (ActA) from day 2 to day 5. Arrows indicate time points of analysis. c , Relative mRNA expression of ME markers during differentiation of WT, BraKO, EoKO, and double knock-out (dKO) cells measured by qRT-PCR in a 5-day time-course showing the complete absence of ME differentiation in dKO cells. Error bars indicate SEM. p-values for differences of mean expression between WT and dKO samples were calculated by Student’s t-test. *:p≤0.05; **:p≤0.01; ***: p≤0.001; ****: p≤0.0001. d , Immunofluorescence (IF) staining of definitive endoderm (SOX17 and FOXA2) and mesoderm (FOXC2) markers at day 4 of differentiation. Scale bars 100 μm. e , Heatmap showing clustered groups (I to V) of downregulated genes (adjusted p-value≤0.05, log 2 (FC)≤-2.5) in dKO compared to WT cells at day 5 of differentiation analyzed by RNA-seq. Scale indicates centered scaled counts normalized by library size and gene-wise dispersion. Important genes in each group are indicated to the right. f , Gene ontology (GO) enrichment analysis of downregulated genes in dKO compared to WT cells indicating the significantly reduced signatures of ME developmental programs in dKO cells. p-values for each term are indicated.

Article Snippet: EBs were subsequently transferred into 60 mm non-adhesive dishes and allowed to further differentiate in ESGRO Complete Basal medium with 30 ng/ml human recombinant ActivinA (ActA, R&D systems).

Techniques: Knock-In, Expressing, Knock-Out, Quantitative RT-PCR, Immunofluorescence, Staining, RNA Sequencing Assay, Dispersion